Whole Effluent Toxicity Testing

Whole Effluent Toxicity (WET) refers to the aggregate toxic effect to aquatic organisms from all pollutants contained in a facility’s wastewater (effluent). It is one way we implement the Clean Water Act’s prohibition of the discharge of toxic pollutants in toxic amounts. WET tests measure wastewater’s effects on specific test organisms’ ability to survive, grow and reproduce.

The WET methods listed below are specified in 40 CFR 136.3, Table I A. WET test methods consist of exposing living aquatic organisms (plants, vertebrates and invertebrates) to various concentrations of a sample of wastewater, usually from a facility’s effluent stream. WET tests are used by the National Pollutant Discharge Elimination System (NPDES) permitting authority to determine whether a facility’s permit will need to include WET requirements.

WET Testing Services

Fathead minnow (Pimephales promelas) Larval Survival and Growth Test (EPA 1000.0/OECD 210.0)
Fathead minnow Embryo-Larval Survival and Teratogenicity Test (EPA 1001.0)
Ceriodaphnia dubia Survival and Reproduction Test (EPA 1002.0)
Green Alga, Selenastrum capricornutum, Growth Test (EPA 1003.0/OECD 201.0)
Fathead minnow (EPA 2000.0/OECD 203.0)
Ceriodaphnia dubia (EPA 2002.0/OECD 202.0)
Daphnia pulex (EPA 2021.0/OECD 202.0)
Daphnia magna (EPA 2021.0/OECD 202.0/OECD 211.0)
Cyprinodon variegatus (EPA 2004.0/OECD 203.0)
Americamysis bahia, Mysid Shrimp (EPA 2006.0/OECD 203.0)
Menidia beryllina (EPA 2007.0/OECD 203.0)
Aquatic Toxicity Testing of Lubricants (ASTM D6081-98)
Toxicity Identification Evaluations (TIE)/Toxicity Reduction Evaluations (TRE)

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We work with your schedule, not ours. No penalty for cancelling or rescheduling tests.